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readystrip™ 142 immobilized ph gradient (ipg) strip (17 cm, ph 3–10 nonlinear)  (Bio-Rad)


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    Bio-Rad readystrip™ 142 immobilized ph gradient (ipg) strip (17 cm, ph 3–10 nonlinear)
    Readystrip™ 142 Immobilized Ph Gradient (Ipg) Strip (17 Cm, Ph 3–10 Nonlinear), supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/readystrip+ipg+strip+17/10__1016_slash_j__jcs__2020__102935-91-7-20?v=Bio-Rad
    Average 90 stars, based on 1 article reviews
    readystrip™ 142 immobilized ph gradient (ipg) strip (17 cm, ph 3–10 nonlinear) - by Bioz Stars, 2026-06
    90/100 stars

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    a N. caninum -based immunoblot showing the immuno-reactivity of α-rNcNTPase antibodies against parasite extracts. A main band of approximately 64 kDa was detected. b NcNTPase-immunome profile of the tachyzoite stage of the Nc-Liv isolate. Proteins were separated along a non-linear pH gradient (pH <t>3–10</t> <t>NL,</t> <t>IPG</t> strips; 17 cm) in the first dimension and on a 10 % polyacrylamide gel in the second dimension. Following transfer to PVDF membranes, a polyclonal rabbit antiserum raised against rNcNTPase was used. The 2-DE immunoblot was analyzed with the PD-Quest software. Arrows indicate the detected protein spots. c Immunogold TEM of N. caninum tachyzoites labelled with the α-rNcNTPase antibody. The images show in detail the apical region and the dense granules in longitudinal and cross sections. The NcNTPase is clearly associated with dense granules ( arrow ). Dense granules (dg), micronemes (mic), rhoptries (rh) and the nucleus (nuc) are indicated on the micrographs. Scale-bars : 0.5 μm
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    a N. caninum -based immunoblot showing the immuno-reactivity of α-rNcNTPase antibodies against parasite extracts. A main band of approximately 64 kDa was detected. b NcNTPase-immunome profile of the tachyzoite stage of the Nc-Liv isolate. Proteins were separated along a non-linear pH gradient (pH <t>3–10</t> <t>NL,</t> <t>IPG</t> strips; 17 cm) in the first dimension and on a 10 % polyacrylamide gel in the second dimension. Following transfer to PVDF membranes, a polyclonal rabbit antiserum raised against rNcNTPase was used. The 2-DE immunoblot was analyzed with the PD-Quest software. Arrows indicate the detected protein spots. c Immunogold TEM of N. caninum tachyzoites labelled with the α-rNcNTPase antibody. The images show in detail the apical region and the dense granules in longitudinal and cross sections. The NcNTPase is clearly associated with dense granules ( arrow ). Dense granules (dg), micronemes (mic), rhoptries (rh) and the nucleus (nuc) are indicated on the micrographs. Scale-bars : 0.5 μm
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    a N. caninum -based immunoblot showing the immuno-reactivity of α-rNcNTPase antibodies against parasite extracts. A main band of approximately 64 kDa was detected. b NcNTPase-immunome profile of the tachyzoite stage of the Nc-Liv isolate. Proteins were separated along a non-linear pH gradient (pH <t>3–10</t> <t>NL,</t> <t>IPG</t> strips; 17 cm) in the first dimension and on a 10 % polyacrylamide gel in the second dimension. Following transfer to PVDF membranes, a polyclonal rabbit antiserum raised against rNcNTPase was used. The 2-DE immunoblot was analyzed with the PD-Quest software. Arrows indicate the detected protein spots. c Immunogold TEM of N. caninum tachyzoites labelled with the α-rNcNTPase antibody. The images show in detail the apical region and the dense granules in longitudinal and cross sections. The NcNTPase is clearly associated with dense granules ( arrow ). Dense granules (dg), micronemes (mic), rhoptries (rh) and the nucleus (nuc) are indicated on the micrographs. Scale-bars : 0.5 μm
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    a N. caninum -based immunoblot showing the immuno-reactivity of α-rNcNTPase antibodies against parasite extracts. A main band of approximately 64 kDa was detected. b NcNTPase-immunome profile of the tachyzoite stage of the Nc-Liv isolate. Proteins were separated along a non-linear pH gradient (pH <t>3–10</t> <t>NL,</t> <t>IPG</t> strips; 17 cm) in the first dimension and on a 10 % polyacrylamide gel in the second dimension. Following transfer to PVDF membranes, a polyclonal rabbit antiserum raised against rNcNTPase was used. The 2-DE immunoblot was analyzed with the PD-Quest software. Arrows indicate the detected protein spots. c Immunogold TEM of N. caninum tachyzoites labelled with the α-rNcNTPase antibody. The images show in detail the apical region and the dense granules in longitudinal and cross sections. The NcNTPase is clearly associated with dense granules ( arrow ). Dense granules (dg), micronemes (mic), rhoptries (rh) and the nucleus (nuc) are indicated on the micrographs. Scale-bars : 0.5 μm
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    a N. caninum -based immunoblot showing the immuno-reactivity of α-rNcNTPase antibodies against parasite extracts. A main band of approximately 64 kDa was detected. b NcNTPase-immunome profile of the tachyzoite stage of the Nc-Liv isolate. Proteins were separated along a non-linear pH gradient (pH <t>3–10</t> <t>NL,</t> <t>IPG</t> strips; 17 cm) in the first dimension and on a 10 % polyacrylamide gel in the second dimension. Following transfer to PVDF membranes, a polyclonal rabbit antiserum raised against rNcNTPase was used. The 2-DE immunoblot was analyzed with the PD-Quest software. Arrows indicate the detected protein spots. c Immunogold TEM of N. caninum tachyzoites labelled with the α-rNcNTPase antibody. The images show in detail the apical region and the dense granules in longitudinal and cross sections. The NcNTPase is clearly associated with dense granules ( arrow ). Dense granules (dg), micronemes (mic), rhoptries (rh) and the nucleus (nuc) are indicated on the micrographs. Scale-bars : 0.5 μm
    Readystrip Ipg Strip (17 Ph 5–7, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a N. caninum -based immunoblot showing the immuno-reactivity of α-rNcNTPase antibodies against parasite extracts. A main band of approximately 64 kDa was detected. b NcNTPase-immunome profile of the tachyzoite stage of the Nc-Liv isolate. Proteins were separated along a non-linear pH gradient (pH 3–10 NL, IPG strips; 17 cm) in the first dimension and on a 10 % polyacrylamide gel in the second dimension. Following transfer to PVDF membranes, a polyclonal rabbit antiserum raised against rNcNTPase was used. The 2-DE immunoblot was analyzed with the PD-Quest software. Arrows indicate the detected protein spots. c Immunogold TEM of N. caninum tachyzoites labelled with the α-rNcNTPase antibody. The images show in detail the apical region and the dense granules in longitudinal and cross sections. The NcNTPase is clearly associated with dense granules ( arrow ). Dense granules (dg), micronemes (mic), rhoptries (rh) and the nucleus (nuc) are indicated on the micrographs. Scale-bars : 0.5 μm

    Journal: Parasites & Vectors

    Article Title: The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress

    doi: 10.1186/s13071-016-1620-4

    Figure Lengend Snippet: a N. caninum -based immunoblot showing the immuno-reactivity of α-rNcNTPase antibodies against parasite extracts. A main band of approximately 64 kDa was detected. b NcNTPase-immunome profile of the tachyzoite stage of the Nc-Liv isolate. Proteins were separated along a non-linear pH gradient (pH 3–10 NL, IPG strips; 17 cm) in the first dimension and on a 10 % polyacrylamide gel in the second dimension. Following transfer to PVDF membranes, a polyclonal rabbit antiserum raised against rNcNTPase was used. The 2-DE immunoblot was analyzed with the PD-Quest software. Arrows indicate the detected protein spots. c Immunogold TEM of N. caninum tachyzoites labelled with the α-rNcNTPase antibody. The images show in detail the apical region and the dense granules in longitudinal and cross sections. The NcNTPase is clearly associated with dense granules ( arrow ). Dense granules (dg), micronemes (mic), rhoptries (rh) and the nucleus (nuc) are indicated on the micrographs. Scale-bars : 0.5 μm

    Article Snippet: Isoelectric focusing (IEF) was performed on 17 cm-ReadyStrip™ IPG Strips pH 3–10 NL (Bio-Rad) with a Protean IEF cell system (Bio-Rad).

    Techniques: Western Blot, Software